Watanabe T, Itoh S
Department of Clinical Nutrition, Faculty of Health Science, Suzuka University of Medical Science and Technology, Mie, Japan.
Cancer Immunol Immunother. 1997 Jun;44(4):216-20. doi: 10.1007/s002620050376.
The mean survival rates of female BDF1 mice transplanted intravenously (i.v.) with murine L1210 leukemia cells were significantly prolonged by intraperitoneal (i.p.) pretreatment (before i.v. transplantation) or by i.p. pre- and post-treatment (before and after the i.v. transplantation) with heat-killed Actinobacillus suis cells ATCC 15557 (AS 15557) alone, as compared with untreated L1210-leukemia-cell-bearing control mice. However, significant prolongation of the mean survival rates was not elicited by the i.p. post-treatment with AS 15557 alone. When 5-fluorouracil (5-FU) was applied i.p. to mice receiving post-treatment with AS 15557 alone, the mean survival rates of the L1210-leukemia-cell-bearing mice were significantly prolonged. The antileukemic action of AS 15557, alone or in combination with 5-FU, against L1210 leukemia was superior to that of a streptococcal preparation (OK-432) and was almost the same as of bacillus Calmette-Guérin with or without 5-FU. The results suggest the possibility that the synergism of AS 15557 in combination with 5-FU may be dependent on the relationship between the indirect immunological function of AS 15557 and the direct cytotoxic action of 5-FU on L1210 leukemia cells.
与未处理的携带L1210白血病细胞的对照小鼠相比，通过腹腔内（i.p.）预处理（静脉内移植前）或通过单独用热灭活的猪放线杆菌细胞ATCC 15557（AS 15557）进行腹腔内预处理和后处理（静脉内移植前后），可显著延长静脉内（i.v.）移植鼠L1210白血病细胞的雌性BDF1小鼠的平均存活率。然而，单独用AS 15557进行腹腔内后处理并未引起平均存活率的显著延长。当将5-氟尿嘧啶（5-FU）腹腔内应用于仅接受AS 15557后处理的小鼠时，携带L1210白血病细胞的小鼠的平均存活率显著延长。AS 15557单独或与5-FU联合对L1210白血病的抗白血病作用优于链球菌制剂（OK-432），并且与卡介苗单独或联合5-FU的作用几乎相同。结果提示AS 15557与5-FU联合的协同作用可能取决于AS 15557的间接免疫功能与5-FU对L1210白血病细胞的直接细胞毒性作用之间的关系。
