Gomes O A, de Souza R R, Liberti E A
Department of Anatomy, University of São Paulo, Brazil.
Gerontology. 1997;43(4):210-7. doi: 10.1159/000213852.
We have examined the number of nerve cells present in the myenteric plexus of the human large intestine using a nonhistochemical method (Giemsa) in laminar preparations of the muscularis externa in two groups of subjects aged 20-35 and over 65 years. The collagen and elastic system related fibers in the myenteric ganglia were also qualitatively evaluated. The total number of neurons decreased in the old subjects by over 37%. The perikaryal area of most of the neurons in both the young and old subjects fell from 101 to 200 microns2. A ganglionic capsule was present and was thicker in the ganglia from the old subjects as were the septa within the ganglia. Both collagen and elastic system fibers were more numerous in the ganglia from the old subjects. We conclude that the decrease in neuron density with age is accompanied by an apparent increase in the fibrous components of the myenteric ganglia.
我们采用非组织化学方法（吉姆萨染色），在两组年龄分别为20 - 35岁和65岁以上受试者的大肠肌层外膜层状标本中，检查了人结肠肌间神经丛中神经细胞的数量。还对肌间神经节中与胶原和弹性系统相关的纤维进行了定性评估。老年受试者的神经元总数减少了37%以上。年轻和老年受试者中大多数神经元的核周面积从101平方微米降至200平方微米。存在神经节被膜，老年受试者神经节中的被膜更厚，神经节内的隔膜也是如此。老年受试者神经节中的胶原纤维和弹性系统纤维都更多。我们得出结论，随着年龄增长神经元密度降低的同时，肌间神经节的纤维成分明显增加。
