Savidge J R, Bristow D R
University of Manchester, School of Biological Sciences, Division of Neuroscience, UK.
Neuroreport. 1997 May 27;8(8):1877-82. doi: 10.1097/00001756-199705260-00017.
Some AMPA receptors are permeable to Ca2+. It has been suggested that cultured rat cerebellar granule cells express Ca(2+)-permeable AMPA receptors, but their distribution at a single cell level is unknown. We report that AMPA (in the presence of cyclothiazide) induced Ca2+ entry (measured by Mn2+ quench of fura-2 fluorescence) and intracellular Ca2+ increases in cerebellar granule cells in the absence of extracellular Na+, supporting the presence of Ca(2+)-permeable AMPA receptors. Analysis of intracellular Ca2+ signals in single cells demonstrated a heterogeneous distribution of Ca(2+)-permeable AMPA receptors.
一些α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体对Ca2+具有通透性。有人提出，培养的大鼠小脑颗粒细胞表达对Ca2+具有通透性的AMPA受体，但它们在单细胞水平上的分布尚不清楚。我们报告称，在没有细胞外Na+的情况下，AMPA（在环噻嗪存在时）诱导小脑颗粒细胞内Ca2+内流（通过fura-2荧光的Mn2+淬灭来测量）以及细胞内Ca2+增加，这支持了对Ca2+具有通透性的AMPA受体的存在。对单细胞内Ca2+信号的分析表明，对Ca2+具有通透性的AMPA受体分布不均一。
