小鼠视网膜视锥细胞中肌营养不良蛋白定位的免疫细胞化学研究。
Immunocytochemical study of dystrophin localization in cone cells of mouse retinas.
作者信息
Ueda H, Kato Y, Baba T, Terada N, Fujii Y, Tsukahara S, Ohno S
机构信息
Department of Anatomy, Yamanashi Medical University, Tamaho, Japan.
出版信息
Invest Ophthalmol Vis Sci. 1997 Jul;38(8):1627-30.
PURPOSE
Previously, the authors reported that dystrophin was observed under the rod cell membranes in rat retinas. However, it was not determined whether dystrophin is located in cone cells. In the current study, the authors clarify dystrophin localization in cone cells of mouse retinas.
METHODS
Immunoblotting, confocal laser scanning microscopy, and immunoelectron microscopy were used to investigate retinal dystrophin with a monoclonal antibody raised against the human dystrophin C-terminus.
RESULTS
Immunoblotting analysis showed some immunoreactive bands from retinal extracts. Confocal images indicated two different immunostaining patterns: One was a tiny dot, and the other was a larger, aggregated dot. Immunoelectron microscopy revealed that retinal dystrophin was localized in cone cells as well as in rod cells.
CONCLUSIONS
Retinal dystrophin is a common component of cone and rod cells and probably is related to the physiological function of photoreceptor cells.
目的
此前，作者报道在大鼠视网膜的视杆细胞膜下观察到了肌营养不良蛋白。然而，尚未确定肌营养不良蛋白是否位于视锥细胞中。在当前研究中，作者阐明了小鼠视网膜视锥细胞中肌营养不良蛋白的定位。
方法
使用免疫印迹、共聚焦激光扫描显微镜和免疫电子显微镜，用针对人肌营养不良蛋白C末端产生的单克隆抗体研究视网膜肌营养不良蛋白。
结果
免疫印迹分析显示视网膜提取物中有一些免疫反应条带。共聚焦图像显示出两种不同的免疫染色模式：一种是微小的点，另一种是较大的聚集点。免疫电子显微镜显示视网膜肌营养不良蛋白位于视锥细胞以及视杆细胞中。
结论
视网膜肌营养不良蛋白是视锥细胞和视杆细胞的共同成分，可能与光感受器细胞的生理功能有关。
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