Barradas C, Dunel-Erb S, Lignon J
Centre d'Ecologie et Physiologie Energétiques, Centre National de la Recherche Scientifique, Strasbourg, France.
Arch Physiol Biochem. 1997 Feb;105(1):38-44. doi: 10.1076/apab.105.1.38.13140.
A new method is described that allows in vitro perfusion and transepithelial electrical potential measurements of a single filament (3-5 mm long; 200 microns in diameter) isolated from the podobranch of the crayfish Astacus leptodactylus. An electrophysiological study was carried out on the preparation to validate this technique. The good physiological quality of the isolated filament preparation has been established and results of continuous measurements of the potential difference under two perfusion conditions are reported. Filaments were perfused with Van Harreveld physiological saline inside and either with Van Harreveld saline or artificial fresh water outside. Large potential differences up to 150 mV between inside and outside of the filament were recorded. When filaments were symmetrically perfused, the behavior of the electrical potential difference allowed two populations of filaments to be distinguished.
本文描述了一种新方法，该方法能够对从小龙虾细足螯虾的 podobranch 分离出的单根细丝（长 3 - 5 毫米；直径 200 微米）进行体外灌注和跨上皮电势测量。对该制剂进行了电生理研究以验证此技术。已确定分离细丝制剂具有良好的生理质量，并报告了在两种灌注条件下电势差的连续测量结果。细丝内部用范·哈雷维尔德生理盐水灌注，外部则用范·哈雷维尔德生理盐水或人工淡水灌注。记录到细丝内外的电势差高达 150 mV。当细丝进行对称灌注时，电势差的行为使两种细丝群体得以区分。
