El Housni H, Radulescu A, Lecocq R, Dumont J E, Christophe D
IRIBHN, Université Libre de Bruxelles, Faculté de Médecine, Brussels, Belgium.
Biochim Biophys Acta. 1997 Jun 26;1352(3):249-52. doi: 10.1016/s0167-4781(97)00073-0.
Calcyphosine, initially identified as thyroid protein p24, is a calcium-binding protein containing four EF-hand domains. It was first cloned and characterized in the dog and corresponds to R2D5 antigen in rabbit. Using the canine calcyphosine cDNA sequence as a probe, we have isolated its human counterpart from a thyroid cDNA library. The two sequences display a high degree of conservation, both at nucleotide and deduced amino acid levels. Sequence comparison with other proteins showed that the closest homologue of calcyphosine is the crustacean CCBP-23 protein. Northern blot analysis revealed that calcyphosine messenger RNA is much less abundant in human than in canine thyrocytes. Western blot experiments indicated that the amount of protein is also dramatically reduced in man compared to dog.
钙磷蛋白最初被鉴定为甲状腺蛋白p24，是一种含有四个EF手型结构域的钙结合蛋白。它最初是在犬类中克隆和表征的，对应于兔中的R2D5抗原。利用犬类钙磷蛋白cDNA序列作为探针，我们从甲状腺cDNA文库中分离出了其人类对应物。这两个序列在核苷酸和推导的氨基酸水平上都显示出高度的保守性。与其他蛋白质的序列比较表明，钙磷蛋白最接近的同源物是甲壳类CCBP - 23蛋白。Northern印迹分析显示，钙磷蛋白信使RNA在人类甲状腺细胞中的丰度远低于犬类。Western印迹实验表明，与犬类相比，人类中该蛋白的量也显著减少。
