Asturias J A, Arilla M C, Bartolomé B, Martínez J, Martínez A, Palacios R
R&D Department, IFIDESA-ARISTEGUI, Bilbao, Spain.
Biochim Biophys Acta. 1997 Jun 26;1352(3):253-7. doi: 10.1016/s0167-4781(97)00061-4.
Three cDNA clones encoding timothy grass pollen profilin (Phl p 11) were newly isolated. Comparison of the sequences of four cDNA clones, including a previously isolated clone, showed a low level of polymorphism. Isoelectrofocusing of highly purified timothy grass profilin indicated the existence of at least five isoforms. One recombinant profilin showed similar immunological properties to natural timothy grass profilin. Tertiary structure of Phleum pratense profilin was obtained by homology-based molecular modeling.
新分离出了三个编码梯牧草花粉肌动蛋白结合蛋白（Phl p 11）的cDNA克隆。对包括先前分离的克隆在内的四个cDNA克隆的序列进行比较，结果显示多态性水平较低。对高度纯化的梯牧草肌动蛋白结合蛋白进行等电聚焦分析表明，至少存在五种同工型。一种重组肌动蛋白结合蛋白表现出与天然梯牧草肌动蛋白结合蛋白相似的免疫学特性。通过基于同源性的分子建模获得了草地早熟禾肌动蛋白结合蛋白的三级结构。
