A型和B型爱泼斯坦-巴尔病毒核抗原6均与RBP-2N相互作用。

Both A type and B type Epstein-Barr virus nuclear antigen 6 interact with RBP-2N.

作者信息

Young D B, Krauer K, Kienzle N, Sculley T

机构信息

Queensland Institute of Medical Research, EBV Unit, Bancroft Centre, Herston Queensland, Australia.

出版信息

J Gen Virol. 1997 Jul;78 ( Pt 7):1671-4. doi: 10.1099/0022-1317-78-7-1671.

DOI:10.1099/0022-1317-78-7-1671

PMID:9225044

Abstract

Using the yeast two-hybrid system, Epstein-Barr virus nuclear antigen 6A (EBNA6A) was found to interact with the RBP-2N isoform of RBP-J kappa. The interaction of EBNA6A and EBNA6B with RBP-2N was compared and the results indicated that EBNA6B was less efficient at interacting with RBP-2N than was EBNA6A. Deletion mutation analysis of EBNA6A identified a region involved in the interaction with RBP-2N, while analysis of RBP-2N identified a domain which interacts with EBNA6A. The region of RBP-2N to which EBNA6A binds has previously been shown to interact with EBNA2.

摘要

利用酵母双杂交系统，发现爱泼斯坦-巴尔病毒核抗原6A（EBNA6A）与RBP-Jκ的RBP-2N亚型相互作用。对EBNA6A和EBNA6B与RBP-2N的相互作用进行了比较，结果表明，EBNA6B与RBP-2N相互作用的效率低于EBNA6A。对EBNA6A进行缺失突变分析，确定了一个与RBP-2N相互作用的区域，而对RBP-2N的分析确定了一个与EBNA6A相互作用的结构域。EBNA6A结合的RBP-2N区域先前已被证明与EBNA2相互作用。