Tanaka M, Yamazaki Y, Kondo E, Hattori M, Tsushita K, Utsumi M
Department of Clinical Research, Nagoya National Hospital, Japan.
Leuk Res. 1997 May;21(5):465-8. doi: 10.1016/s0145-2126(97)00114-8.
Hydroxyurea rarely produces a complete cytogenetic remission in patients with chronic myelogenous leukemia (CML). In this report, we describe one case of the CML patient who achieved complete cytogenetic remission (no Ph chromosome in 20-25 metaphase cells) by treatment with hydroxyurea alone. By the fluorescent in situ hybridization (FISH) methodology using bcr/abl specific translocation probe, sequential bone marrow specimens from the patient showed the characteristic 9;22 translocation at a higher rate (9-10%) than the normal control range (2.49-4.88%) at the time of complete cytogenetic remission. Thus, it is suggested that FISH is a more sensitive method to detect the bcr/abl fusion gene than conventional cytogenetic analysis for the detection of minimal residual disease in CML.
羟基脲很少能使慢性粒细胞白血病（CML）患者实现完全细胞遗传学缓解。在本报告中，我们描述了1例CML患者，该患者仅通过羟基脲治疗就实现了完全细胞遗传学缓解（20 - 25个中期细胞中无费城染色体）。通过使用bcr/abl特异性易位探针的荧光原位杂交（FISH）方法，该患者连续的骨髓标本显示，在完全细胞遗传学缓解时，特征性的9;22易位发生率（9 - 10%）高于正常对照范围（2.49 - 4.88%）。因此，提示对于检测CML微小残留病，FISH是一种比传统细胞遗传学分析更敏感的检测bcr/abl融合基因的方法。
