Ryabov E V, Alieva I B, Uzbekov R E, Uzbekova S V, Vorobjev L A
Belozersky Institute of Physico-Chemical Biology, Moscow State University.
Membr Cell Biol. 1997;10(5):503-13.
Rabbit polyclonal antibodies were raised against the C-terminal fragment (amino acid residues 318-451) of human gamma-tubulin. These antibodies were used to stain cultured cells of various tissues (epithelium, nervous tissue, fibroblasts) from different animals (human, monkey, pig, rat, kangaroo rat, mouse, hamster, chicken, triton). The antibodies specifically stained centrioles in the interphase and mitotic cells of mammals, but not birds (chicken) or amphibians (newt). In the interphase cells, centrioles were stained as a pair of dots (or as a double dot) in 96-97% of the cells. The distances between the maternal and filial centrioles varied in different cultures. Procentrioles were stained in certain cells, but with less intensity than mature centrioles. In mitotic cells, the antibodies revealed two spots corresponding to two mitotic poles. The spots in mitosis were significantly larger than the interphase dots, but the staining was more faint. In spontaneous tripolar mitoses, only two poles were stained. Thus, it was shown that, on the one hand, gamma-tubulin is associated with centrioles irrespective of whether or not they serve as the microtubule organizing centres and, on the other hand, gamma-tubulin might not be an essential component of the microtubule organizing centres.
制备了针对人γ-微管蛋白C末端片段（氨基酸残基318 - 451）的兔多克隆抗体。这些抗体用于对来自不同动物（人、猴、猪、大鼠、更格卢鼠、小鼠、仓鼠、鸡、蝾螈）的各种组织（上皮组织、神经组织、成纤维细胞）的培养细胞进行染色。这些抗体特异性地对哺乳动物间期和有丝分裂细胞中的中心粒进行染色，但对鸟类（鸡）或两栖动物（蝾螈）无效。在间期细胞中，96 - 97%的细胞中，中心粒被染成一对点（或双点）。母代和子代中心粒之间的距离在不同培养物中有所不同。原中心粒在某些细胞中被染色，但强度低于成熟中心粒。在有丝分裂细胞中，抗体显示出对应于两个有丝分裂极的两个斑点。有丝分裂中的斑点明显大于间期的点，但染色更淡。在自发的三极有丝分裂中，只有两个极被染色。因此，一方面表明，无论中心粒是否作为微管组织中心，γ-微管蛋白都与中心粒相关；另一方面表明，γ-微管蛋白可能不是微管组织中心的必需成分。
