Yegutkin G G
Institute of Radiobiology, Belorussian Academy of Sciences, Minsk, Republic of Belarus.
Membr Cell Biol. 1997;10(5):515-20.
The extent of solubilization and the structure of rat liver and adipose plasma membranes after treatment with nonionic detergent Triton X-100 were studied. The concentration of Triton X-100 varied from 0.005 to 0.050% (0.26-2.6 mg/mg membrane protein). The excimerization of the pyrene fluorescent probe and the relative vibronic band intensities in the pyrene monomer fluorescence spectrum were measured to evaluate the membrane lipid bilayer fluidity and polarity. The data on the aqueous pyrene fluorescence in the presence of Triton X-100 showed that formation of micellar aggregates occurred at detergent concentrations of over 0.015%. This value is a crucial factor in the detergent action on the membrane structure: both intensive extraction of plasma membrane proteins and fluidization of the lipid bilayer were observed only at Triton X-100 concentration over 0.015%. However, Triton X-100 did not exert any action on the polarity of the membrane hydrophobic regions.
研究了用非离子去污剂Triton X-100处理后大鼠肝脏和脂肪细胞质膜的增溶程度及结构。Triton X-100的浓度在0.005%至0.050%之间变化（0.26 - 2.6毫克/毫克膜蛋白）。测量芘荧光探针的激基缔合物形成以及芘单体荧光光谱中的相对振动带强度，以评估膜脂双层的流动性和极性。在Triton X-100存在下的水相芘荧光数据表明，在去污剂浓度超过0.015%时会形成胶束聚集体。该值是去污剂对膜结构作用的关键因素：仅在Triton X-100浓度超过0.015%时，才观察到质膜蛋白的大量提取和脂双层的流化。然而，Triton X-100对膜疏水区域的极性没有任何作用。
