Stuhne-Sekalec L, Stanacev N Z
Can J Biochem. 1977 Nov;55(11):1159-65. doi: 10.1139/o77-173.
Translocation of membrane-bound labelled lipids from guinea pig liver microsomal to mitochondrial membranes was studied. When microsomal membranes containing known amounts and composition of spin-labelled radioactive lipids were incubated with unlabelled mitochondrial membranes, reisolated mitochondria contained spin- and radioactive-lipids in an amount which could not be accounted for by the microsomal contamination of reisolated mitochondria, establishing therefore the translocation of labelled lipids from microsomal to mitochondrial membranes. The effect of addition of crude 105000 X g supernatant on the translocation was studied. The translocation of labelled lipids in aged membranes was also described. The rate of loss of paramagnetism in microsomal and reisolated mitochondrial membranes was measured and found to be different, supporting the conclusion that the translocation of labelled lipids between membranes took place. Data from these studies suggested that the translocatndrial membranes probably occurred. Furthermore, our results suggest that the mechanism by which lipid translocation takes place does not necessarily involve soluble cytosol proteins. In view of experimental data, possible mechanisms for the translocation of lipids between biological membranes were discussed.
研究了豚鼠肝微粒体中膜结合标记脂质向线粒体膜的转运。当含有已知量和组成的自旋标记放射性脂质的微粒体膜与未标记的线粒体膜一起孵育时，重新分离的线粒体中所含的自旋脂质和放射性脂质的量，无法用重新分离的线粒体中的微粒体污染来解释，因此确定了标记脂质从微粒体向线粒体膜的转运。研究了添加105000×g粗上清液对转运的影响。还描述了老化膜中标记脂质的转运情况。测量了微粒体膜和重新分离的线粒体膜中顺磁性丧失的速率，发现两者不同，这支持了标记脂质在膜之间发生转运的结论。这些研究的数据表明，标记脂质可能在线粒体膜之间发生了转运。此外，我们的结果表明，脂质转运发生的机制不一定涉及可溶性胞质溶胶蛋白。鉴于实验数据，讨论了生物膜之间脂质转运的可能机制。
