Zunić J, Stavljenić-Rukavina A, Granić P, Zanić-Matanić D, Husedzinović I, Romić Z, Holosek A, Belavić Z
Department of Anesthesiology and Intensive Care, County Hospital Karlovac, Croatia.
Coll Antropol. 1997 Jun;21(1):327-34.
The aim of this randomized study was to examine changes in vitamin E concentration in female subjects (age 30-60, ASA I) after cholecystectomy and halothane (N = 16) or isoflurane (N = 16) anaesthesia. Vitamin E concentration was measured two days before, and then one, five and twenty-four hours and four days after surgery. High-pressure liquid chromatography was used for its determination. Simultaneously activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyltransferase (GGT) were determined.
ANOVA, Tukay HSD test. The research has been accepted by the Drugs Committee of the Karlovac County Hospital. Preoperative vitamin E concentrations in the halothane group were 8.69 +/- 2.35 micrograms/L, median 8.67 micrograms/L and in the isoflurane group 9.43 +/- 2.4 micrograms/L, median 9.08 micrograms/L. Statistically lower vitamin E concentrations compared with preoperative values were noted one hour (P < 0.05), 5 hours (P < 0.01), 24 hours (P < 0.01), as well as 4 days (P < 0.01) after the operation. The lowest vitamin E concentrations were noted 24 hours after the operation with statistically insignificantly higher values in the isoflurane group (halothane group 5.98 +/- 2.08 micrograms/L, isoflurane group 6.58 +/- 1.51 micrograms/L). Analyzing enzyme (ALT, AST and GGT) pre- and postoperative values, no statistically significant differences between the investigated groups and during the time were observed. Statistically significant differences were found between individual measurement times, with no statistical significance of the differences between the halothane and isoflurane groups. It seems that neither the difference in halothane and isoflurane biotransformation nor their distinct effect on perfusion of some organs are the determining factors in post-operative changes in vitamin E concentration.
本随机研究的目的是检测胆囊切除术后，接受氟烷（N = 16）或异氟烷（N = 16）麻醉的女性受试者（年龄30 - 60岁，ASA I级）体内维生素E浓度的变化。在手术前两天，以及术后1小时、5小时、24小时和4天测量维生素E浓度。采用高压液相色谱法进行测定。同时测定丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、γ-谷氨酰转移酶（GGT）的活性。
方差分析、图基 Honestly Significant Difference 检验。该研究已获卡尔洛瓦茨县医院药物委员会批准。氟烷组术前维生素E浓度为8.69±2.35微克/升，中位数为8.67微克/升；异氟烷组术前维生素E浓度为9.43±2.4微克/升，中位数为9.08微克/升。术后1小时（P < 0.05）、5小时（P < 0.01）、24小时（P < 0.01）以及4天（P < 0.01）时，维生素E浓度与术前值相比在统计学上降低。术后24小时维生素E浓度最低，异氟烷组的值在统计学上略高（氟烷组5.98±2.08微克/升，异氟烷组6.58±1.51微克/升）。分析术前和术后酶（ALT、AST和GGT）的值，在研究组之间以及不同时间未观察到统计学上的显著差异。在各个测量时间之间发现了统计学上的显著差异，氟烷组和异氟烷组之间的差异无统计学意义。似乎氟烷和异氟烷生物转化的差异及其对某些器官灌注的不同影响都不是术后维生素E浓度变化的决定因素。
