埃格特维德病毒RNA的纯化及生化特性

Purification and biochemical properties of Egtved viral RNA.

作者信息

Robin J, Rodrigue A

出版信息

Can J Microbiol. 1977 Oct;23(10):1489-91. doi: 10.1139/m77-219.

Abstract

32P-labeled Egtved virus RNA was released from highly purified virus by phenol-SDS extraction. The single-stranded nature of the RNA was demonstrated by (1) its buoyant density of 1.69 g/cm3 in Cs2-SO4, (2) its susceptibility to digestion by pancreatic ribonuclease in either 1 X SSC or 0.01 X SSC (standard saline citrate), (3) its base composition (29.3% C, 23.6% A, 14.5% U. 32.6% G). This Egtved virus is different from the other rhabdoviruses since the base composition of its genomic RNA is lower in its composition of A + U. Such a result could have possible taxonomic implications concerning the orignin and evolution of Egtved Virus relative to the other known rhabdoviruses.

摘要

通过苯酚 - SDS 提取法从高度纯化的病毒中释放出用³²P 标记的艾格特维德病毒 RNA。RNA 的单链性质通过以下几点得以证明：（1）其在 Cs₂SO₄ 中的浮力密度为 1.69 g/cm³；（2）在 1×SSC 或 0.01×SSC（标准柠檬酸盐缓冲液）中对胰核糖核酸酶消化敏感；（3）其碱基组成（29.3% C、23.6% A、14.5% U、32.6% G）。这种艾格特维德病毒与其他弹状病毒不同，因为其基因组 RNA 的碱基组成中 A + U 的含量较低。这样的结果可能对艾格特维德病毒相对于其他已知弹状病毒的起源和进化具有分类学意义。