Phillips D J, Capparella M, Neue U D, el Fallah Z
Waters Corporation, Milford, MA 01757, USA.
J Pharm Biomed Anal. 1997 Jun;15(9-10):1389-95. doi: 10.1016/s0731-7085(96)02049-3.
The need for fast and efficient separations of complex samples such as pharmaceuticals and biologicals led to the development of fast, efficient, and reproducible 3.5 microns columns. Separations using 3.5 microns column are 30-50% faster at equal plate-count compared to 5 microns columns. The results show that the 3.5 microns columns (100 mm length) give the same efficiency and resolution of drug impurities as the 5 microns columns (150 mm length). For many analytical methods, switching to 3.5 microns columns saves time and reduces costs. Separation methodologies using 5 microns columns are easily modified to accommodate 3.5 microns columns of the same chemistry because efficiency, resolution and sensitivity remain the same. It is shown that 3.5 microns columns have lifetimes comparable to 5 microns columns of the same brand.
对药物和生物制品等复杂样品进行快速高效分离的需求，促使了快速、高效且可重现的3.5微米色谱柱的发展。与5微米色谱柱相比，在相同塔板数下，使用3.5微米色谱柱进行分离的速度要快30%-50%。结果表明，3.5微米色谱柱（100毫米长度）与5微米色谱柱（150毫米长度）对药物杂质具有相同的分离效率和分辨率。对于许多分析方法而言，改用3.5微米色谱柱可节省时间并降低成本。使用5微米色谱柱的分离方法很容易进行修改，以适应相同化学性质的3.5微米色谱柱，因为分离效率、分辨率和灵敏度保持不变。结果表明，3.5微米色谱柱的使用寿命与同一品牌的5微米色谱柱相当。
