Lebed O I, Zhirnov V V, Luik A I, Braver-Chernobulskaya B S, Yartsev V G, Kuzin V G
Institute of Pharmacology and Toxicology, Academy of Medical Sciences of Ukraine, Kyiv.
Ukr Biokhim Zh (1978). 1996 Nov-Dec;68(6):68-76.
beta, beta-Dichlorodiethylamine is proved not to induce structural disturbances in phosphatidylcholine liposomes and erythrocyte membranes which is registered by fluorescence methods. Methyl-beta, beta-dichlorodiethylamine and metaxylyl-beta, beta-dichlorodiethylamine cause the increase in microviscosity of lipid bilayer hydrophobic areas in both erythrocyte membranes and liposomes. Besides, polarity of the latter also decreases, and the metaxylyl derivative alkylates nucleophilic centers of phospholipid phosphate groups in liposomes. Erythrocyte membranes, being treated by beta, beta-dichlorodiethylamine derivatives, the increase in the membrane protein hydrophobicity is registered as well as the decrease in their immersion in the lipid bilayer.
β，β-二氯二乙胺经荧光法检测证实不会在磷脂酰胆碱脂质体和红细胞膜中引起结构紊乱。甲基-β，β-二氯二乙胺和间二甲苯-β，β-二氯二乙胺会导致红细胞膜和脂质体中脂质双层疏水区域的微粘度增加。此外，后者的极性也会降低，间二甲苯衍生物会使脂质体中磷脂磷酸基团的亲核中心烷基化。用β，β-二氯二乙胺衍生物处理红细胞膜后，会检测到膜蛋白疏水性增加以及其在脂质双层中的浸入减少。
