Faulkes C G, Abbott D H, O'Brien H P, Lau L, Roy M R, Wayne R K, Bruford M W
Conservation Genetics Group, Institute of Zoology, Zoological Society of London, UK.
Mol Ecol. 1997 Jul;6(7):615-28. doi: 10.1046/j.1365-294x.1997.00227.x.
Patterns of genetic structure in eusocial naked mole-rat populations were quantified within and among geographically distant populations using multilocus DNA fingerprinting and mitochondrial DNA (mtDNA) sequence analysis. Individuals within colonies were genetically almost monomorphic, sharing the same mtDNA control region haplotype and having coefficients of band sharing estimated from DNA fingerprints ranging from 0.93 to 0.99. Family analysis of a hybrid captive colony of naked mole-rats with increased levels of genetic variability using multilocus DNA fingerprinting gave results consistent with Mendelian inheritance, and has revealed for the first time that multiple paternity can occur. In a survey of wild colonies from Ethiopia, Somalia and locations in northern and southern Kenya, we have examined mtDNA control region sequence variation in 42 individuals from 15 colonies, and together with multilocus DNA fingerprinting and mtDNA cytochrome-b sequence analysis in selected individuals have shown that these populations show considerable genetic divergence. Most of the variance in sequence divergence was found to be between geographical locations (phi ct = 0.68) and there was a significant correlation between sequence divergence and geographical separation of haplotypes. Six colonies from Mtito Andei in southern Kenya shared the same control region haplotype, suggesting a recent common maternal ancestor. In contrast, out of four colonies at Lerata in north Kenya, three haplotypes were identified, and phylogenetic analysis suggests that this area may be a zone where two distinct lineages are in close proximity. Genetic distances were maximal between Ethiopian and southern Kenyan populations at 5.8% for cytochrome-b, and are approaching interspecific values seen between other Bathyergids.
利用多位点DNA指纹图谱和线粒体DNA（mtDNA）序列分析，对地理上相距遥远的群居裸鼹鼠种群内部和种群之间的遗传结构模式进行了量化。群体内的个体在基因上几乎是单态的，共享相同的mtDNA控制区单倍型，并且从DNA指纹图谱估计的条带共享系数在0.93至0.99之间。使用多位点DNA指纹图谱对遗传变异性增加的裸鼹鼠杂交圈养群体进行家系分析，结果与孟德尔遗传一致，并且首次揭示了多父本现象可以发生。在对来自埃塞俄比亚、索马里以及肯尼亚北部和南部地区的野生群体的调查中，我们检测了来自15个群体的42个个体的mtDNA控制区序列变异，并且结合对选定个体的多位点DNA指纹图谱和mtDNA细胞色素b序列分析表明，这些种群显示出相当大的遗传差异。序列差异中的大部分变异被发现存在于地理位置之间（φct = 0.68），并且单倍型的序列差异与地理隔离之间存在显著相关性。肯尼亚南部Mtito Andei的六个群体共享相同的控制区单倍型，这表明它们最近有共同的母系祖先。相比之下，在肯尼亚北部Lerata的四个群体中，鉴定出了三种单倍型，系统发育分析表明该地区可能是两个不同谱系紧密相邻的区域。埃塞俄比亚和肯尼亚南部种群之间的细胞色素b遗传距离最大，为5.8%，并且正在接近其他滨鼠科动物之间的种间值。
