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一种从须鲸历史标本中分离DNA的有效方法。

An effective method for isolating DNA from historical specimens of baleen.

作者信息

Rosenbaum H C, Egan M G, Clapham P J, Brownell R L, Desalle R

机构信息

Molecular Systematics Laboratory, American Museum of Natural History, New York, NY 10024, USA.

出版信息

Mol Ecol. 1997 Jul;6(7):677-81. doi: 10.1046/j.1365-294x.1997.00230.x.

DOI:10.1046/j.1365-294x.1997.00230.x
PMID:9226948
Abstract

DNA was isolated from an early twentieth century museum specimen of northern right whale baleen. A system of stringent controls and a novel set of cetacean specific primers eliminated contamination from external sources and ensured the authenticity of the results. Sequence analysis revealed that there were informative nucleotide positions between the museum specimen and extant members of the population and closely related species. The results indicate that museum specimens of baleen can be used to assess historical genetic population structure of the great whales.

摘要

从一头20世纪早期的北露脊鲸鲸须博物馆标本中分离出了DNA。一套严格的对照系统和一组全新的鲸类特异性引物消除了外部来源的污染,并确保了结果的真实性。序列分析表明,该博物馆标本与该种群现存成员及近缘物种之间存在信息丰富的核苷酸位点。结果表明,鲸须博物馆标本可用于评估大型鲸鱼的历史遗传种群结构。

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