Lin S D, Chai C Y, Chou C K, Tsai C C, Lai C S
Department of Surgery, Chung-Ho Memorial Hospital, Kaohsiung Medical College, Taiwan, Republic of China.
Kaohsiung J Med Sci. 1997 May;13(5):301-7.
In a rabbit model, transplantation of largely expanded microskin grafts (expansion ratio 25 X) were studied. In Group I, the autologous microskin grafts were transplanted onto the full-thickness skin defect on the back of a rabbit and were overlaid with Biobrane. In Group II, the autologous microskin grafts were mixed with allogeneic microdermal substance (expansion ratio 25 X) before the transplantation. The percentage of re-epithelialized area to the total grafted wound was analyzed by means of computerized planimetric analyses of photographs of the grafted wounds on days 11 and 13. Biopsies of the grafted wounds were done. On day 11, the mean percentage of Group I was 72.4% and that of Group II was 82.7%. On day 13, they were 82.3% in Group I and 92.3% in Group II. In Group II, the allogeneic dermal tissue did not cause obvious rejection in the neoepithelium. Histological features showed the existence of allogeneic dermal tissue in the grafted wound. The adding of largely expanded allogeneic dermal substance to autologous microskin grafts in Group II provided better circumstances than that in Group I for re-epithelialization of autologous mciroskin grafts.
在兔模型中，研究了大幅扩张的微型皮肤移植物（扩张比例为25倍）的移植情况。在第一组中，将自体微型皮肤移植物移植到兔背部的全层皮肤缺损处，并用生物膜覆盖。在第二组中，自体微型皮肤移植物在移植前与同种异体微型真皮物质（扩张比例为25倍）混合。通过对移植伤口在第11天和第13天的照片进行计算机平面测量分析，分析再上皮化面积占总移植伤口面积的百分比。对移植伤口进行活检。在第11天，第一组的平均百分比为72.4%，第二组为82.7%。在第13天，第一组为82.3%，第二组为92.3%。在第二组中，同种异体真皮组织在新上皮中未引起明显排斥反应。组织学特征显示移植伤口中存在同种异体真皮组织。第二组中在自体微型皮肤移植物中添加大幅扩张的同种异体真皮物质，为自体微型皮肤移植物的再上皮化提供了比第一组更好的条件。
