Ferguson D J
Cancer Res. 1977 Dec;37(12):4367-71.
Previous experiments demonstrating a reduction of tumorigenicity by roughening the surfaces of plastics implanted in rodents, or by increasing the pore size of cellulose filter implants, were repeated with observations on cellular attachment to these objects and to filters strengthened and made impermeable by bonding to plastic. Round 13-mm discs of methylmethacrylate implanted S.C. in A/BiF/F50 + mice produced sarcomas in 12 percent of mice at 64 weeks. Tumor incidence increased to 60 percent (p less than 0.001) in mice receiving discs to which cellulose filters with pore sizes of 0.025 to 0.1 micrometer were bonded. No tumors occurred with discs covered by 0.45-micrometer filters, followed up to 83 weeks. Vinyl coverslips 15 mm square also produced no sarcomas when covered by 0.45 micrometer filters; plain vinyl produced sarcomas in 40 percent of mice at 64 weeks (p less than 0.001). Sanding of vinyl surfaces reduced tumorigenicity (p less than 0.05). Permeability, fragility, and storage capacity of filters are apparently not related to tumorigenicity. Surface roughness probably is related. Cells, mostly macrophages, were densely and uniformly attached to nontumorigenic surfaces from 24 hr to 2 years after inplantation but were distinctly fewer and not uniformly distrubuted on tumorigenic surfaces. Topology favoring attachment was inherent in 0.45-mum filters and was produced in plastic by gouging irregular excavations 10 to 15 micrometer deep.
先前的实验表明，通过使植入啮齿动物体内的塑料表面粗糙化，或增加纤维素滤膜植入物的孔径，可以降低致瘤性。这些实验被重复进行，并观察细胞对这些物体以及通过与塑料结合而得到强化并变得不可渗透的滤膜的附着情况。将13毫米的甲基丙烯酸甲酯圆盘皮下植入A/BiF/F50 +小鼠体内，在64周时，12%的小鼠产生了肉瘤。在接受粘贴了孔径为0.025至0.1微米纤维素滤膜的圆盘的小鼠中，肿瘤发生率增至60%（p小于0.001）。用孔径为0.45微米的滤膜覆盖的圆盘，随访至83周未出现肿瘤。边长为15毫米的乙烯基盖玻片在被0.45微米滤膜覆盖时也未产生肉瘤；普通乙烯基盖玻片在64周时使40%的小鼠产生了肉瘤（p小于0.001）。对乙烯基表面进行打磨降低了致瘤性（p小于0.05）。滤膜的渗透性、易碎性和储存能力显然与致瘤性无关。表面粗糙度可能与之相关。从植入后24小时到2年，细胞（主要是巨噬细胞）密集且均匀地附着在非致瘤性表面，但在致瘤性表面上明显较少且分布不均。有利于附着的拓扑结构是0.45微米滤膜所固有的，并且通过在塑料上挖出10至15微米深的不规则凹坑而产生。
