Mazza G, el Idrissi M E, Coutelier J P, Corato A, Elson C J, Pfau C J, Day M J
Department of Pathology and Microbiology, School of Medical Sciences, University Walk, University of Bristol, UK.
Immunology. 1997 Jun;91(2):239-45. doi: 10.1046/j.1365-2567.1997.00242.x.
C3HeB/FeJ mice infected with the docile strain of lymphocytic choriomeningitis virus (LCMV-d) develop a persistent infection with a transient haemolytic anaemia. Immunoglobulin can be eluted from the red blood cells (RBC) of these mice but it cannot be detected on the RBC by a conventional antiglobulin test. The present study demonstrates that RBC from such mice bear erythrocyte autoantibodies which are predominantly of the IgG2a subclass, with lower levels of autoantibodies of the IgG1, IgG2b and IgG3 subclasses. To identify the target antigen the autoantibodies were eluted from the RBC of LCMV-infected mice. The eluted autoantibody bound to intact normal RBC and precipitated a 105000 MW component that corresponds to murine Band 3 protein. A monoclonal antibody derived from mice infected with LCMV-d also precipitated mouse Band 3, and reacted specifically by enzyme-linked immunosorbent assay against a purified preparation of Band 3. This study has shown that in C3H mice infected with LCMV-d which develop autoimmune haemolytic anaemia, the target autoantigen is erythrocyte membrane Band 3.
感染温和型淋巴细胞性脉络丛脑膜炎病毒（LCMV-d）的C3HeB/FeJ小鼠会发生持续性感染，并伴有短暂的溶血性贫血。免疫球蛋白可从这些小鼠的红细胞（RBC）中洗脱出来，但通过传统的抗球蛋白试验在红细胞上检测不到。本研究表明，此类小鼠的红细胞带有红细胞自身抗体，主要为IgG2a亚类，IgG1、IgG2b和IgG3亚类的自身抗体水平较低。为了鉴定靶抗原，从感染LCMV的小鼠红细胞中洗脱自身抗体。洗脱的自身抗体与完整的正常红细胞结合，并沉淀出一种105000MW的成分，该成分对应于小鼠带3蛋白。源自感染LCMV-d小鼠的单克隆抗体也沉淀出小鼠带3，并通过酶联免疫吸附测定法与纯化的带3制剂发生特异性反应。这项研究表明，在感染LCMV-d并发生自身免疫性溶血性贫血的C3H小鼠中，靶自身抗原是红细胞膜带3。
