Senden N H, Timmer E D, de Bruïne A, Wagenaar S S, Van de Velde H J, Roebroek A J, Van de Ven W J, Broers J L, Ramaekers F C
Department of Molecular Cell Biology and Genetics, University Maastricht, The Netherlands.
J Pathol. 1997 May;182(1):13-21. doi: 10.1002/(SICI)1096-9896(199705)182:1<13::AID-PATH804>3.0.CO;2-Z.
Neuroendocrine-specific protein (NSP)-reticulons are endoplasmic reticulum-associated protein complexes, which have been identified as markers for neuroendocrine differentiation. In this study, the expression of two members of the family of NSP-reticulons, NSP-A and NSP-C, have been investigated in different types of lung cancer and compared with the expression patterns of five conventional neuroendocrine markers, the neural cell adhesion molecule (NCAM), synaptophysin, chromogranin A, Leu-7, and neurofilament proteins. NSP-A and NSP-C antibodies were reactive with most carcinoid tumour and small cell lung carcinoma (SCLC) cases, while atypical carcinoid tumours showed a variable expression. In the total group of neuroendocrine tumours, a high concordance of expression was found between NSP-A and NSP-C, while their expression correlated well with NCAM and synaptophysin positivity. Chromogranin A, Leu-7, and neurofilament proteins were shown to be expressed to a limited extent in these neuroendocrine tumours. In a selected group of non-SCLCs known to exhibit neuroendocrine features, NSP-A expression was detected at much higher frequency than NSP-C. In virtually all NSP-A positive cases, this expression was associated with one or more of the other neuroendocrine markers. NSP-A expression showed a stronger correlation with conventional neuroendocrine markers than NCAM. In detecting neuroendocrine differentiation in non-SCLC, NSP-A is more sensitive than synaptophysin, chromogranin A, Leu-7, and neurofilament proteins. It is concluded that NSP-reticulons are valuable markers in the diagnosis of neuroendocrine differentiation in non-SCLC and should be used in conjunction with NCAM.
神经内分泌特异性蛋白（NSP）-网织蛋白是与内质网相关的蛋白复合物，已被确定为神经内分泌分化的标志物。在本研究中，对NSP-网织蛋白家族的两个成员NSP-A和NSP-C在不同类型肺癌中的表达进行了研究，并与五种传统神经内分泌标志物（神经细胞黏附分子（NCAM）、突触素、嗜铬粒蛋白A、Leu-7和神经丝蛋白）的表达模式进行了比较。NSP-A和NSP-C抗体与大多数类癌肿瘤和小细胞肺癌（SCLC）病例反应，而非典型类癌肿瘤则表现出可变表达。在神经内分泌肿瘤的总体组中，发现NSP-A和NSP-C之间的表达高度一致，而它们的表达与NCAM和突触素的阳性密切相关。嗜铬粒蛋白A、Leu-7和神经丝蛋白在这些神经内分泌肿瘤中的表达有限。在一组已知具有神经内分泌特征的非小细胞肺癌（NSCLC）中，NSP-A表达的检测频率远高于NSP-C。在几乎所有NSP-A阳性病例中，这种表达与一种或多种其他神经内分泌标志物相关联。NSP-A表达与传统神经内分泌标志物的相关性比NCAM更强。在检测非小细胞肺癌中的神经内分泌分化时，NSP-A比突触素、嗜铬粒蛋白A、Leu-7和神经丝蛋白更敏感。结论是，NSP-网织蛋白是诊断非小细胞肺癌神经内分泌分化的有价值标志物，应与NCAM联合使用。
