Viacava P, Naccarato A G, Collecchi P, Ménard S, Castronovo V, Bevilacqua G
Institute of Pathology, University of Pisa, Italy.
J Pathol. 1997 May;182(1):36-44. doi: 10.1002/(SICI)1096-9896(199705)182:1<36::AID-PATH802>3.0.CO;2-W.
Laminin is a glycoprotein of the basement membrane (BM), involved in a variety of normal and pathological cellular events including tumour invasion and metastasis. Cells bind laminin through different types of receptor. The 67-kD laminin receptor (67LR) is a cell-surface protein which binds laminin with high affinity. 67LR expression has been shown to increase in neoplastic cells, compared with normal tissues, and 67LR seems to play an important role during the first steps of neoplastic progression. In this study, 67LR expression was analysed during the morphological phases of breast cancer progression from normal tissue to invasive carcinoma. A total of 506 formalin-fixed, paraffin-embedded normal breast structures and lesions were stained by immunohistochemistry usign the MLuC5 monoclonal antibody, which is specific for 67LR. The results show that in normal breast and in any kind of breast lesion, myoepithelial and endothelial cells express 67LR. While 67LR is not seen in the epithelium of normal breast, cysts, adenosis, and benign tumours, it is expressed in the epithelial cells of several hyperplasias and carcinomas in situ, both ductal and lobular, as well as in all invasive carcinomas. The 67LR-positive cell subpopulation expands from hyperplastic lesions to invasive carcinoma, suggesting that 67LR could be related to the induction and progression of breast cancer.
层粘连蛋白是基底膜（BM）的一种糖蛋白，参与包括肿瘤侵袭和转移在内的多种正常和病理细胞事件。细胞通过不同类型的受体与层粘连蛋白结合。67-kD层粘连蛋白受体（67LR）是一种细胞表面蛋白，它以高亲和力结合层粘连蛋白。与正常组织相比，肿瘤细胞中67LR的表达已被证明会增加，并且67LR似乎在肿瘤进展的最初阶段发挥重要作用。在本研究中，分析了乳腺癌从正常组织进展到浸润性癌的形态学阶段中67LR的表达情况。使用对67LR具有特异性的MLuC5单克隆抗体，通过免疫组织化学对总共506个福尔马林固定、石蜡包埋的正常乳腺结构和病变进行染色。结果表明，在正常乳腺以及任何类型的乳腺病变中，肌上皮细胞和内皮细胞表达67LR。虽然在正常乳腺、囊肿、腺病和良性肿瘤的上皮中未见67LR，但在一些导管和小叶的增生以及原位癌的上皮细胞中表达，以及在所有浸润性癌中均有表达。67LR阳性细胞亚群从增生性病变扩展到浸润性癌，表明67LR可能与乳腺癌的诱导和进展有关。
