Lauweryns J M, Cokelaere M, Deleersynder M, Liebens M
Cell Tissue Res. 1977 Sep 5;182(4):425-40. doi: 10.1007/BF00219827.
Neonatal rabbit neuro-epithelial bodies (NEB) were investigated under various experimental conditions with light microscopy, microspectrography, morphometry and electron microscopy. (1) Hypoxia causes a decreased amine fluorescence intensity and an increased secretory exocytosis of dense core vesicles (DCV). Otherwise the NEB appear structurally normal. (2) Hypercapnia also produces a decreased fluorescence and an increased exocytosis; ultrastructurally, however, the dense core of DCV fragmentizes. (3) Hyperoxia does not appear to affect significantly either fluorescence or exocytosis. (4) The uptake of biogenic amines such as 5-HTP and L-DOPA was demonstrated by fluorometry and electron microscopy. (5) Reserpine, on the other hand, provokes an amine depletion with a decrease of the NEB fluorescence and an ultrastructural palor of the DCV. (6) Intratracheally administered nicotine is accompanied by a decreased fluorescence and a distinct exocytosis of fragmented DCV. The reaction of NEB to hypoxia and hypercapnia suggests that these corpuscles could be intrapulmonary chemoreceptors (in addition to the classically known central and peripheral chemoreceptors), inducing a reflex reaction through the liberation of DCV at the corpuscular sensible nerve endings and via the CNS. In addition, they may subserve a local intrapulmonary effect by modulating directly the hypoxic and hypercapnic pulmonary vasoconstriction and thus the V/Q ratio.
在各种实验条件下，利用光学显微镜、显微光谱仪、形态测量学和电子显微镜对新生兔神经上皮小体（NEB）进行了研究。（1）缺氧导致胺荧光强度降低，致密核心囊泡（DCV）的分泌性胞吐作用增强。除此之外，NEB在结构上看起来正常。（2）高碳酸血症也会导致荧光降低和胞吐作用增强；然而，在超微结构上，DCV的致密核心会碎片化。（3）高氧似乎对荧光或胞吐作用均无显著影响。（4）通过荧光测定法和电子显微镜证实了生物胺如5-羟色氨酸和左旋多巴的摄取。（5）另一方面，利血平会引发胺耗竭，导致NEB荧光降低以及DCV超微结构变淡。（6）气管内给予尼古丁会伴随着荧光降低以及碎片化DCV的明显胞吐作用。NEB对缺氧和高碳酸血症的反应表明，这些小体可能是肺内化学感受器（除了经典的中枢和外周化学感受器之外），通过在小体感觉神经末梢释放DCV并经由中枢神经系统诱导反射反应。此外，它们可能通过直接调节缺氧和高碳酸血症性肺血管收缩从而调节V/Q比值来发挥局部肺内效应。
