Pérez L F, Tutor J C
Laboratorio Central, Hospital General de Galicia-Clínico Universitario, Santiago de Compostela, Spain.
Eur J Clin Chem Clin Biochem. 1997 Jun;35(6):445-52. doi: 10.1515/cclm.1997.35.6.445.
Activity of serum/plasma beta-N-acetylhexosaminidase (EC 3.2.1.52) was determined by means of a continuous spectrophotometric method using 3,3'-dichlorophenylsulphonphthaleinyl-N-acetyl-beta-D-glucosaminid e as substrate, with very satisfactory results. Incubation of an undiluted aliquot (1 ml) of samples at 52 degrees C for 8 hours with an adjusted pH 5.5-6.0 provoked only the inactivation of isoenzyme A, thus allowing the evaluation of beta-N-acetylhexosaminidase isoenzyme composition. In 25 serum samples from control subjects and pregnant women, a good correlation between the percentage of isoenzyme B obtained by this procedure and the fluorimetric assay of O'Brien et al. (New Engl J Med 1970; 273:15-20) was found (r = 0.983, S(yx) = 1.51), with no statistically significant difference between the means (43.2 vs 42.8%). In 84 healthy adult subjects, an average value of 30.3% for the proportion of isoenzyme B was obtained, with an interval of 25.4-35.0%, in agreement with results reported by other authors.
采用连续分光光度法，以3,3'-二氯苯基磺酞基-N-乙酰-β-D-氨基葡萄糖苷为底物，测定血清/血浆β-N-乙酰己糖胺酶（EC 3.2.1.52）的活性，结果非常令人满意。将1 ml未稀释的样品等分试样在52℃、pH值调整为5.5 - 6.0的条件下孵育8小时，仅会导致同工酶A失活，从而能够评估β-N-乙酰己糖胺酶的同工酶组成。在25份来自对照受试者和孕妇的血清样本中，通过该方法获得的同工酶B百分比与奥布赖恩等人的荧光测定法（《新英格兰医学杂志》1970年；273:15 - 20）之间存在良好的相关性（r = 0.983，S(yx) = 1.51），均值之间无统计学显著差异（43.2%对42.8%）。在84名健康成年受试者中，同工酶B的比例平均值为30.3%，范围为25.4 - 35.0%，与其他作者报道的结果一致。
