Rodríguez V, Centeno M, Ulrich M
Section of Immunology II, Instituto de Biomedicina, Caracas, Venezuela.
Parasite Immunol. 1996 Jul;18(7):341-5. doi: 10.1046/j.1365-3024.1996.d01-113.x.
American cutaneous leishmaniasis (ACL) presents a spectrum of clinical and immunological manifestations. Since the nature of the cellular response appears to play a fundamental role in determining the characteristics of the immunoglobulin isotype of specific antibody responses, we have compared the relative levels of specific antibodies of the four IgG isotypes against Leishmania in sera from patients with different clinical manifestations of ACL. Using a specific antibody capture assay, significant levels of antibodies of the IgG1, 2 and 3 isotypes were detected in localized cutaneous leishmaniasis (LCL); the average level of IgG4 antibodies was low and they were not detected in 10/20 sera. Sera from muco-cutaneous leishmaniasis (MCL) gave a comparatively strong IgG1 response. Sera from diffuse cutaneous leishmaniasis (DCL), the rare form characterized by antigen-specific anergy of cell-mediated immunity, showed highly significant levels of IgG4 antibodies compared to antibody levels of this isotype in the other groups; IgG1 and IgG2 levels were also elevated. Based on other studies of the relationship between the IgG isotype response and cell-mediated immunity, these results confirm a Th1-like CD4+ T cell response in LCL and MCL and a significant Th2-like response in DCL.
美洲皮肤利什曼病（ACL）呈现出一系列临床和免疫表现。由于细胞反应的性质似乎在决定特异性抗体反应的免疫球蛋白同种型特征方面起着基本作用，我们比较了ACL不同临床表现患者血清中针对利什曼原虫的四种IgG同种型特异性抗体的相对水平。使用特异性抗体捕获试验，在局限性皮肤利什曼病（LCL）中检测到显著水平的IgG1、2和3同种型抗体；IgG4抗体的平均水平较低，且在20份血清中的10份中未检测到。黏膜皮肤利什曼病（MCL）患者的血清产生了相对较强的IgG1反应。弥漫性皮肤利什曼病（DCL）是一种以细胞介导免疫的抗原特异性无反应性为特征的罕见形式，与其他组中该同种型的抗体水平相比，其IgG4抗体水平显著升高；IgG1和IgG2水平也升高。基于其他关于IgG同种型反应与细胞介导免疫之间关系的研究，这些结果证实LCL和MCL中存在类似Th1的CD4 + T细胞反应，而DCL中存在显著的类似Th2的反应。
