Shlykov S G, Babich L G, Robenets N A, Kosterin S A
Ukr Biokhim Zh (1978). 1996 Sep-Oct;68(5):25-34.
Uterotonic peptide hormone oxytocin has been studied for its effect on ATP-dependent accumulation of Ca2+ nonsensitive to the effect of ruthenium red (10 microM) that is potentiated by Ca2+-precipitating anion - oxalate. That was studied in experiments made on the suspension of myometrium cells from estrogenized rats processed by digitonin (0.1 mg/ml) with the aim permeabilization of plasmatic membrane. The preliminary processing of intact myocytes by oxytocin solution (final concentration 100 mM) to permeabilization of plasmatic membrane causes partial inhibition (stimulated by oxalate by 25-30% (0-10 mM) of Mg2+, ATP-dependent accumulation of Ca2+ in nonmitochondrion intracellular calcium depot of perforates smooth-muscled cells. The value of the component of power-dependent accumulation of Ca2+, that is inhibited by oxytocin, increased with both the incubation time and oxalate concentration. Thapsigargin and cyclopiasonium acid, selective inhibitors of calcium pump of endo(sarco)plasmatic reticulum, when used in saturation concentration (50 mM and 10 mM, respectively), completely inhibit the component of Mg2+, ATP-dependent accumulation of Ca2+ in nonmitochondrial intracellular calcium depot that is stimulated with oxalate (< 10 mM) and inhibited with oxytocin (100 mM). It is concluded that oxytocin partially inhibits Mg2+, ATP-dependent calcium pump of myometrium cell endoplasmic reticulum.
子宫收缩肽激素催产素已被研究其对三磷酸腺苷（ATP）依赖性钙离子积累的影响，这种积累对钌红（10微摩尔）的作用不敏感，且会因钙离子沉淀阴离子——草酸盐而增强。这是在对经洋地黄皂苷（0.1毫克/毫升）处理的雌激素化大鼠子宫肌层细胞悬浮液进行的实验中进行研究的，目的是使质膜通透化。用催产素溶液（终浓度100毫摩尔）对完整的肌细胞进行预处理以使质膜通透化，会导致部分抑制（草酸盐刺激下，在穿孔平滑肌细胞的非线粒体细胞内钙库中，镁离子、ATP依赖性钙离子积累被抑制25 - 30%（0 - 10毫摩尔））。被催产素抑制的钙离子功率依赖性积累成分的值，会随着孵育时间和草酸盐浓度的增加而增加。毒胡萝卜素和环匹阿尼酸，分别为内质（肌）网钙泵的选择性抑制剂，当以饱和浓度（分别为50毫摩尔和10毫摩尔）使用时，会完全抑制草酸盐（< 10毫摩尔）刺激且被催产素（100毫摩尔）抑制的非线粒体细胞内钙库中镁离子、ATP依赖性钙离子积累成分。得出的结论是，催产素部分抑制子宫肌层细胞内质网的镁离子、ATP依赖性钙泵。
