Basinskiĭ S N, Grigorenko A A, Krasnogorskaia V N, Solomina E V
Department of Eye Diseases of Amur State Medical Academy, Blagoveshchensk.
Vestn Oftalmol. 1997 Mar-Apr;113(2):8-10.
The morphology of histological slices of tissue at the site of direct revascularization of the choroid was assessed in various periods (15 days, 1, 3, and 6 months) after surgery. The surgical method was as follows: a collagen hemostatic sponge, a strip of subconjunctival tissue, and tissue of the external rectal muscle were put onto an exposed 4 x 4 mm site of the choroid and closed with an episcleral flap fixed with nodular sutures without pulling. Experiments were carried out on 13 rabbits (26 eyes). Direct revascularization of the choroid was performed in all animals. The animals were sacrificed by air embolism 15 days, 1, 3, and 6 months after surgery. Vascular filling was carried out. The eyes were enucleated, paraffin slices were prepared from tissues at the site of operation, and the slices were stained with hematoxylin-eosin, tholuidine blue, and after Van Gieson. After direct revascularization of the choroid with episcleral, muscular, and subconjunctival tissue and collagen sponge numerous full-value vascular formations are seen at the site of the intervention as early as 3 months after surgery; these vascular formations connect the extraorbital tissues rich in vessels with the vascular coating; the number of newly formed vessels increases by 6 months postoperation. The results of experiments give good grounds for clinical use of the method.
在手术后的不同时期(15天、1个月、3个月和6个月),对脉络膜直接血管重建部位的组织切片形态进行了评估。手术方法如下:将一块胶原止血海绵、一条结膜下组织条和直肠外肌组织置于暴露的4×4毫米脉络膜部位,并用结节缝线固定的巩膜瓣封闭,不牵拉。对13只兔子(26只眼)进行了实验。所有动物均进行了脉络膜直接血管重建。在手术后15天、1个月、3个月和6个月,通过空气栓塞处死动物,进行血管灌注。摘除眼球,从手术部位的组织制备石蜡切片,切片用苏木精-伊红、甲苯胺蓝染色,并在范吉森染色后观察。在用巩膜、肌肉和结膜下组织以及胶原海绵对脉络膜进行直接血管重建后,早在手术后3个月,在干预部位就可见到大量有功能的血管形成;这些血管形成将富含血管的眶外组织与血管膜连接起来;术后6个月新形成的血管数量增加。实验结果为该方法的临床应用提供了充分的依据。