用十二烷基硫酸钠对临床标本进行预处理不适用于分枝杆菌生长指示管培养法。
Pretreatment of clinical specimens with sodium dodecyl (lauryl) sulfate is not suitable for the mycobacteria growth indicator tube cultivation method.
作者信息
Pfyffer G E, Welscher H M, Kissling P
机构信息
Swiss National Center for Mycobacteria, Department of Medical Microbiology, University of Zurich.
出版信息
J Clin Microbiol. 1997 Aug;35(8):2142-4. doi: 10.1128/jcm.35.8.2142-2144.1997.
Abstract
When using the Mycobacteria Growth Indicator Tube (MGIT), pretreatment of clinical specimens with N-acetyl-L-cysteine-NaOH is recommended by the manufacturer. Processing of clinical specimens (n = 1,000) with sodium dodecyl (lauryl) sulfate-NaOH resulted in both poor recovery and delayed mean time to detection of acid-fast bacilli. Values were comparable to those obtained on solid media.
摘要
使用分枝杆菌生长指示管（MGIT）时，制造商建议用N-乙酰-L-半胱氨酸-氢氧化钠对临床标本进行预处理。用十二烷基硫酸钠-氢氧化钠处理临床标本（n = 1000），导致抗酸杆菌的回收率低且平均检测时间延迟。这些值与在固体培养基上获得的值相当。
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