Monleón E, Pacheco M C, Luján L, Bolea R, Luco D F, Vargas M A, Alabart J L, Badiola J J, Amorena B
CSIC, Department of Animal Health, Montañana, Zaragoza, Spain.
Vet Microbiol. 1997 Jun 30;57(1):13-28. doi: 10.1016/s0378-1135(97)00080-1.
This work was aimed at studying the effect of maedi-visna virus (MVV) infection in vitro on the ability of sheep cells to adhere to staphylococci (Staphylococcus aureus and Staphylococcus epidermidis), and phagocytose these bacteria. Adherence was studied in sheep choroid plexus cells (SCPC) using an ELISA test and phagocytosis was studied in pulmonary alveolar macrophages (PAM) by chemiluminescence. A 5- and 7-day of in vitro MVV infection resulted in syncytium formation and a significant increased adherence (P < 0.01) of SCPC to bacteria. SCPC endogenous fibronectin was significantly higher (P < 0.01) on days 5 and 7 than on day 0 of MVV infection. A significantly decreased phagocytosis (P < 0.05) was also observed on days 5 and 7 of MVV infection in PAM when compared to MVV-free controls. Comparatively, phagocytosis was highest for S. aureus non-slime producing strains, followed by S. epidermidis, and S. aureus slime producing strains, in that order. Finally, increased expression of both, class I and class II major histocompatibility antigens was also observed in MVV-infected PAM on days 5 and 7, whereas SCPC only demonstrated upregulation of MHC class I. These results, indicative of an alteration of some cell functions in MVV-infected cells, may help to understand interactions between MVV-infected cells and bacteria in simultaneous infections and may provide clues to the possible in vivo interactions of both pathogens.
这项工作旨在研究体外梅迪-维斯纳病毒（MVV）感染对绵羊细胞黏附葡萄球菌（金黄色葡萄球菌和表皮葡萄球菌）以及吞噬这些细菌能力的影响。使用酶联免疫吸附测定（ELISA）试验研究绵羊脉络丛细胞（SCPC）的黏附情况，通过化学发光法研究肺泡巨噬细胞（PAM）的吞噬作用。体外MVV感染5天和7天导致形成多核巨细胞，并且SCPC对细菌的黏附显著增加（P<0.01）。在MVV感染的第5天和第7天，SCPC内源性纤连蛋白显著高于感染第0天（P<0.01）。与未感染MVV的对照相比，在MVV感染的第5天和第7天，PAM的吞噬作用也显著降低（P<0.05）。相比之下，吞噬作用对于不产黏液的金黄色葡萄球菌菌株最高，其次是表皮葡萄球菌，产黏液的金黄色葡萄球菌菌株最低。最后，在MVV感染的第5天和第7天，PAM中I类和II类主要组织相容性抗原的表达均增加，而SCPC仅表现出MHC I类上调。这些结果表明MVV感染细胞中某些细胞功能发生改变，可能有助于理解MVV感染细胞与细菌在同时感染中的相互作用，并可能为两种病原体在体内可能的相互作用提供线索。
