Ishii H, Gobé G C, Joshita T, Kurabayashi Y, Hosomura Y, Kameya T
Department of Pathology, Kitasato University School of Medicine, Sagamihara, Japan.
Arch Histol Cytol. 1997 Jun;60(2):143-52. doi: 10.1679/aohc.60.143.
In recent studies of cycloheximide (CHX)-induced apoptosis in sublines of established Burkitt's lymphoma cell lines (BJA-B) both with and without Epstein-Barr virus (EBV) infection, we noticed two distinct types of apoptosis morphology. In the present paper, we have classified these, and further carried out a statistical analysis of their incidence in untreated and CHX-treated EBV-free (EBV(-)) and EBV-infected (EBV(+)) BJA-B cells.
Both types of apoptosis morphology demonstrated typical nuclear and cytoplasmic condensation. However, "Type 1 apoptotic cells" (AP1) maintained a spherical or ovoid shape, but "Type 2 apoptotic cells" (AP2) were typified by the lobulation of their nuclear and cytoplasmic structures to form "clover leaf" shapes. Statistical analysis of incidence: The numbers of AP1 and AP2 cells were analysed using a chi 2 test, with results as follows: EBV(-) cells underwent AP1 in preference to AP2 (90.5% versus 9.5%) (p < 0.001), whilst EBV(+) cells had comparably more AP2, making AP1 and AP2 approximately equal (49.3% versus 50.7%) (p > 0.1). In EBV(-) cells, treatment with CHX had little effect on the ratios of differing apoptotic morphology. In contrast, in the EBV(+) cells, cell death was altered from AP2 (50.7%-->25.2%) towards AP1(49.3%-->74.8%) (p < 0.001). We propose that cellular proteins known to be associated with EBV infection not only protect the cells from apoptosis, but also affect the phenotype of apoptosis. This knowledge may be useful for defining possible mechanisms of apoptosis induction and/or inhibition in specific models.
在最近对已建立的伯基特淋巴瘤细胞系（BJA - B）的亚系中，使用环己酰亚胺（CHX）诱导细胞凋亡的研究中，我们观察了有无爱泼斯坦 - 巴尔病毒（EBV）感染的两种不同类型的凋亡形态。在本文中，我们对这些形态进行了分类，并进一步对未处理和CHX处理的无EBV（EBV(-)）及感染EBV（EBV(+)）的BJA - B细胞中它们的发生率进行了统计分析。
两种凋亡形态均表现出典型的核及细胞质凝聚。然而，“1型凋亡细胞”（AP1）保持球形或卵形，而“2型凋亡细胞”（AP2）的特征是其核及细胞质结构呈叶状，形成“三叶草叶”形状。发生率的统计分析：使用卡方检验分析AP1和AP2细胞的数量，结果如下：EBV(-)细胞优先发生AP1而非AP2（90.5%对9.5%）（p < 0.001），而EBV(+)细胞中AP2相对较多，使AP1和AP2大致相等（49.3%对50.7%）（p > 0.1）。在EBV(-)细胞中，用CHX处理对不同凋亡形态的比例影响很小。相反，在EBV(+)细胞中，细胞死亡从AP2（50.7% --> 25.2%）转变为AP1（49.3% --> 74.8%）（p < 0.001）。我们提出，已知与EBV感染相关的细胞蛋白不仅保护细胞免于凋亡，还影响凋亡的表型。这一知识可能有助于确定特定模型中凋亡诱导和/或抑制的可能机制。
