Grindley J C, Hargett L K, Hill R E, Ross A, Hogan B L
Department of Cell Biology, Vanderbilt University Medical Center, Nashville, TN 37232-2175, USA.
Mech Dev. 1997 Jun;64(1-2):111-26. doi: 10.1016/s0925-4773(97)00055-5.
Pax6 expression in the diencephalon of the mouse embryo is restricted both antero-posteriorly and dorso-ventrally, with changes in level occurring at prosomere boundaries. Small eye (Pax6Sey-1Neu) mice homozygous for Pax6 mutations have multiple defects in early forebrain development. In the diencephalon of Pax6Sey-1Neu/Pax6Sey-1Neu mice there is an apparent enlargement of the zona limitans (the boundary region between prosomeres p2 and p3), and a blurring of the p1-p2 boundary. PAX6 function is also required for the normal development of the posterior commissure at the midbrain-p1 boundary. In the posterior diencephalon PAX6 appears to regulate its own transcription, and that of Wnt7b. In p2 and p3, ventral markers are expressed more dorsally than normal, and this is accompanied in p3 by a reduction in the size of the zona incerta. Thus, PAX6 is essential for the normal development and regionalization of the diencephalon.
Pax6在小鼠胚胎间脑的表达在前-后轴和背-腹轴上均受到限制，在原节边界处表达水平会发生变化。纯合Pax6突变的小眼（Pax6Sey-1Neu）小鼠在前脑早期发育中存在多种缺陷。在Pax6Sey-1Neu/Pax6Sey-1Neu小鼠的间脑中，界带（原节p2和p3之间的边界区域）明显扩大，p1-p2边界模糊。中脑-p1边界处后连合的正常发育也需要PAX6功能。在间脑后部，PAX6似乎调节其自身以及Wnt7b的转录。在p2和p3中，腹侧标志物的表达比正常情况更靠背侧，并且在p3中伴有未定带大小的减小。因此，PAX6对于间脑的正常发育和区域化至关重要。
