Singh A K, Mishra P, Kashyap R, Chauhan U P
Department of Radiopharmaceuticals, Institute of Nuclear Medicine and Allied Sciences, Lucknow Marg, Delhi, India.
Nucl Med Biol. 1994 Feb;21(2):277-81. doi: 10.1016/0969-8051(94)90020-5.
A kit consisting of reduced human immunoglobulins G (hIgG), methylene diphosphonate, stannous chloride and ascorbic acid has been developed to instantly produce technetium-labelled hIgG of greater than 97% purity and suitable for inflammation foci scintigraphy in patients. The shelf life of the kit when stored at 4-7 degrees C was at least 3 months. 99mTc-hIgG prepared from the kit, when incubated at 37 degrees C for 24 h in physiological saline and human serum was found to degrade by only 7.8 and 4.3%, respectively, thereby indicating high stability of the labelled product. Competitive RIA data did not exhibit loss of immunoreactivity of the hIgG due to its reduction. Blood clearance of the radiopharmaceutical in rabbits exhibited a monophasic exponential pattern. Biodistribution in mice showed uptake by liver (4.93%), kidneys (3.07%) and intestines (2.12%) at 4 h which was reduced to 1.99, 2.18 and 1.93%, respectively at 24 h. Radiolabelled hIgG prepared from the kit was found to be quite satisfactory for inflammation scintigraphy in human patients.
已研发出一种试剂盒，其包含还原型人免疫球蛋白G（hIgG）、亚甲基二膦酸盐、氯化亚锡和抗坏血酸，可即时生产纯度大于97%且适用于患者炎症病灶闪烁显像的锝标记hIgG。该试剂盒在4-7摄氏度储存时的保质期至少为3个月。由该试剂盒制备的99mTc-hIgG，在生理盐水和人血清中于37摄氏度孵育24小时后，发现其降解率分别仅为7.8%和4.3%，从而表明标记产物具有高稳定性。竞争性放射免疫分析数据未显示hIgG因还原而丧失免疫反应性。该放射性药物在兔体内的血液清除呈现单相指数模式。在小鼠体内的生物分布显示，4小时时肝脏摄取率为4.93%，肾脏摄取率为3.07%，肠道摄取率为2.12%，24小时时分别降至1.99%、2.18%和1.93%。由该试剂盒制备的放射性标记hIgG被发现对于人类患者的炎症闪烁显像相当令人满意。
