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[体外测定巨噬细胞吞噬活性的放射性方法]

[Radiometric method of determining the phagocytic activity of macrophages in vitro].

作者信息

Fifi F, Nedialkova M, Serafimov-Dimitrov V

出版信息

Eksp Med Morfol. 1977;16(3):132-6.

PMID:923494
Abstract

The authors describe a radiometric method for determination of phagocytic activity of macrophages, using as an object for phaging hen erythrocytes labeled with 51Cr. The basic principle consists of quantitative determination of phaged cells by means of measuring the activity, found in the phagocytes. The method is described in details and the obtained results in normal mice and such with experimental leucosis NKLI are compared with those, checked by the classical methods for determining phagocytic activity in vivo and in vitro. On the basis of these data the authors indicate the advantage of the method, prepared by them, because it alows objective checking of phagocytosis on a large number of cells and thus increases the possibilities of obtaining significant results.

摘要

作者描述了一种用于测定巨噬细胞吞噬活性的放射性测量方法,该方法以用51Cr标记的鸡红细胞作为吞噬对象。其基本原理是通过测量吞噬细胞中的活性来定量测定被吞噬的细胞。文中详细描述了该方法,并将在正常小鼠以及患有实验性白血病NKLI的小鼠中获得的结果,与通过体内和体外测定吞噬活性的经典方法所检测的结果进行了比较。基于这些数据,作者指出了他们所制备方法的优势,因为它能够对大量细胞的吞噬作用进行客观检测,从而增加了获得显著结果的可能性。

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