Dighiero G, Binet J L
C R Seances Soc Biol Fil. 1979;173(2):213-20.
Seventy one patients with a lymphoid hemopathy, three with agammaglobulinemia and six normal controls were investigated with regard to their blood lymphocyte membrane-associated light chains. Detection and quantitation of antigenic determinants were performed by means of peroxidase-labeled antibodies. Compared to normal controls, values found in chronic lymphocytic leukemia (CLL) were very low (tenfold decrease). The number of antigenic determinants on lymphoid cells from patients with blast crises supervening in CLL, prolymphocytic leukemia, Waldenstrom's macroglobulinemia and Burkitt cell acute leukemia were significantly higher than those seen in patients with CLL. The data obtained in this investigation through quantitative immunocytology constitutes a new parameter for the classification of lymphoid hemopathies and for an approach to their pathogenesis, in particular if the quantity of membrane immunoglobulin correlates with the stage of cell maturation.
对71例淋巴造血系统疾病患者、3例无丙种球蛋白血症患者和6例正常对照者的血淋巴细胞膜相关轻链进行了研究。通过过氧化物酶标记抗体对抗原决定簇进行检测和定量。与正常对照相比，慢性淋巴细胞白血病（CLL）患者的值非常低（降低了10倍）。CLL急变期、原淋巴细胞白血病、华氏巨球蛋白血症和伯基特细胞急性白血病患者的淋巴细胞上的抗原决定簇数量明显高于CLL患者。通过定量免疫细胞学在本研究中获得的数据构成了淋巴造血系统疾病分类及其发病机制研究的一个新参数，特别是如果膜免疫球蛋白的量与细胞成熟阶段相关的话。
