Barbanel G, Borgna J L, Bonnafous J C, Mani J C
Eur J Biochem. 1977 Nov 1;80(2):411-23. doi: 10.1111/j.1432-1033.1977.tb11896.x.
The calculation of equilibrium binding constants for a specific receptor-hormone interaction requires the exact determination of the unbound ligand concentration and the specifically bound concentration at equilibrium. These determinations usually require corrections for the contribution of non-specific binding. We introduce several parameters allowing equilibrium concentrations to be calculated from experimental concentration values; these parameters can be measured for the particular receptor assay procedure used. These parameters are used in a microassay of estradiol-receptor complex by selective retention on DE-81 cellulose paper.
特定受体 - 激素相互作用的平衡结合常数计算需要精确测定平衡时未结合配体的浓度和特异性结合的浓度。这些测定通常需要对非特异性结合的贡献进行校正。我们引入了几个参数，可根据实验浓度值计算平衡浓度；这些参数可针对所使用的特定受体测定程序进行测量。这些参数用于通过在DE - 81纤维素纸上选择性保留来进行雌二醇 - 受体复合物的微量测定。
