Elegado F B, Kim W J, Kwon D Y
Korea Food Research Institute, Kyongki-do, South Korea.
Int J Food Microbiol. 1997 Jun 17;37(1):1-11. doi: 10.1016/s0168-1605(97)00037-8.
The bacteriocin from Pediococcus acidilactici M, designated as Pediocin AcM, was rapidly purified to homogeneity by the pH mediated cell adsorption-desorption method and semi-preparative reversed-phase HPLC. The purification yield was 40.4% and the specific activity was increased by 2450-fold. It gave a single band and a single peak on SDS-PAGE and HPLC analysis, respectively. When subjected to electrospray LC-MS analysis, the protein was found to be highly pure and the molecular weight was determined as 4,618 Da. High concentration of the bacteriocin (> 50 micrograms/ml) showed good resistance to extremes of pH (1-12) and temperature (121 degrees C). Pediocin AcM was deduced to be a monomer with an intra-peptide disulfide bond from the results of reversed-phase analytical HPLC analyses after reduction, oxidation and trypsin digestion. P. acidilactici M inhibited a large number of bacteria, including Staphylococcus aureus, Listeria monocytogenes, Clostridium perfringens, Bacillus coagulans, B. cereus, and Aeromonas hydrophila.
嗜酸乳杆菌M产生的细菌素，命名为嗜酸乳杆菌细菌素AcM，通过pH介导的细胞吸附 - 解吸法和半制备反相高效液相色谱法快速纯化至同质。纯化产率为40.4%，比活性提高了2450倍。在SDS - PAGE和HPLC分析中分别呈现单一条带和单一峰。经电喷雾液相色谱 - 质谱分析，该蛋白质纯度很高，分子量测定为4618 Da。高浓度的细菌素（>50微克/毫升）对极端pH值（1 - 12）和温度（121℃）表现出良好的耐受性。根据还原、氧化和胰蛋白酶消化后的反相分析HPLC分析结果，推断嗜酸乳杆菌细菌素AcM是一种具有肽内二硫键的单体。嗜酸乳杆菌M抑制大量细菌，包括金黄色葡萄球菌、单核细胞增生李斯特菌、产气荚膜梭菌、凝结芽孢杆菌、蜡样芽孢杆菌和嗜水气单胞菌。
