Terada Y, Fukaya T, Takahashi M, Yajima A
Department of Obstetrics and Gynecology, Yamagata Prefectural Central Hospital, Japan.
Mol Hum Reprod. 1996 Nov;2(11):879-81. doi: 10.1093/molehr/2.11.879.
Growth hormone (GH) is believed to be involved in reproductive function. Recent reports demonstrate the presence of the growth hormone receptors (GHR) in reproductive organs and fetal tissues suggesting that the contribution of GH to reproductive phenomena may be mediated through GHR. In this study we assessed the expression of GHR in mouse preimplantation embryos using immunofluorescent staining with a monoclonal antibody directed against GHR. The messenger RNA (mRNA) for GHR was investigated in mouse preimplantation embryo by reverse transcription-polymerase chain reaction (RT-PCR). The anti-GHR monoclonal antibody Mab 263 was detected by immunofluorescent staining after the compaction of the morula stage. GHR mRNA transcript was identified in 8-cell stage embryos. Our findings indicate that GHR is localized in mouse preimplantation embryos, and suggests that GH has a direct effect on preimplantation embryos mediated by GHR.
生长激素（GH）被认为与生殖功能有关。最近的报告表明，生殖器官和胎儿组织中存在生长激素受体（GHR），这表明GH对生殖现象的作用可能是通过GHR介导的。在本研究中，我们使用针对GHR的单克隆抗体进行免疫荧光染色，评估了GHR在小鼠植入前胚胎中的表达。通过逆转录-聚合酶链反应（RT-PCR）研究了小鼠植入前胚胎中GHR的信使核糖核酸（mRNA）。桑椹胚阶段致密化后，通过免疫荧光染色检测到抗GHR单克隆抗体Mab 263。在8细胞期胚胎中鉴定出GHR mRNA转录本。我们的研究结果表明，GHR定位于小鼠植入前胚胎中，这表明GH通过GHR对植入前胚胎有直接作用。
