Barbaro K C, Eickstedt V R, Mota I
Laboratório de Imunopatologia, Instituto Butantan, São Paulo, Brazil.
Toxicon. 1994 Jan;32(1):113-20. doi: 10.1016/0041-0101(94)90027-2.
Antigenic cross-reactivity between the components of venoms from three spiders of the genus Loxosceles, L. gaucho, L. laeta and L. intermedia, was studied. Species-specific antisera were prepared by immunization of rabbits with each venom. Anti-L. gaucho horse hyperimmune serum provided by the Butantan Institute for treatment of accidents with these spiders was also used. Separation by SDS-PAGE showed the existence of many common components in the three antigens. No individual antigen was observed. Analysis of the antisera by ELISA and Western blotting showed cross-reactivity as well as several common bands between the three venoms. The horse anti-L. gaucho venom serum recognized many common proteins when antigens of the other two species were used. Antigens in the range of 33,000-35,000 mol. wt showed most cross-reactivity. Both horse and rabbit anti-venom sera contained antibodies able to neutralize the lethal and dermonecrotic activities of the venom of the three species studied.
研究了洛氏蝎属（Loxosceles）三种蜘蛛——高乔洛氏蝎（L. gaucho）、莱塔洛氏蝎（L. laeta）和中间洛氏蝎（L. intermedia）毒液成分之间的抗原交叉反应性。用每种毒液免疫兔子制备了种特异性抗血清。还使用了布坦坦研究所提供的抗高乔洛氏蝎马超免疫血清来治疗被这些蜘蛛蜇伤的事故。SDS-PAGE分离显示三种抗原中存在许多共同成分。未观察到单个抗原。通过ELISA和Western印迹分析抗血清显示三种毒液之间存在交叉反应以及几条共同条带。当使用其他两个物种的抗原时，马抗高乔洛氏蝎毒液血清识别出许多共同蛋白质。分子量在33,000 - 35,000范围内的抗原显示出最大的交叉反应性。马和兔抗毒液血清均含有能够中和所研究的三种物种毒液的致死和皮肤坏死活性的抗体。
