Takekoshi Y, Sato K, Kanno S, Kawase S, Kiho T, Ukai S
Scientific Investigation Research Laboratory, Gifu Prefecture Police Headquarters, Japan.
Forensic Sci Int. 1997 Jun 6;87(2):85-97. doi: 10.1016/s0379-0738(97)02150-6.
Alkali-catalyzed pyrolysis gas chromatography (PyGC) has been used to identify minute samples of wool fiber. The wool sample to which aqueous sodium hydroxide was added was pyrolyzed in a Curie-point pyrolyzer attached to a gas chromatograph or a gas chromatograph-mass spectrometer. The addition of an aqueous solution of sodium hydroxide increased the production of specific volatile pyrolysis products from the constitutive amino acid residues of wool protein, i.e. acetaldehyde from alanine or proline, isobutyronitrile from valine, 2-methylbutyronitrile from isoleucine, isovaleronitrile from leucine and toluene from phenylalanine. Compared with conventional non-catalyzed PyGC, the alkali-catalyzed PyGC was found to greatly improve the detection limit of wool fiber and make it possible to analyze very minute samples. The alkali-catalyzed PyGC presented here has been shown to be applicable to minute thermally-denatured samples of wool fiber which cannot be identified successfully by morphological inspection using a microscope or by using Fourier-transform infrared microspectroscopy. Furthermore, the present PyGC method was successfully used for several protein samples and was shown to be useful for analysis of proteins other than wool fibers by using different special pyrograms reflecting different amino acid compositions.
碱催化热解气相色谱法（PyGC）已被用于鉴定羊毛纤维的微量样品。将添加了氢氧化钠水溶液的羊毛样品在连接到气相色谱仪或气相色谱 - 质谱仪的居里点热解器中进行热解。氢氧化钠水溶液的添加增加了羊毛蛋白组成氨基酸残基产生特定挥发性热解产物的量，即丙氨酸或脯氨酸产生乙醛、缬氨酸产生异丁腈、异亮氨酸产生2 - 甲基丁腈、亮氨酸产生异戊腈以及苯丙氨酸产生甲苯。与传统的非催化PyGC相比，发现碱催化PyGC大大提高了羊毛纤维的检测限，并使得分析非常微量的样品成为可能。本文介绍的碱催化PyGC已被证明适用于无法通过显微镜形态检查或傅里叶变换红外显微光谱法成功鉴定的微量热变性羊毛纤维样品。此外，目前的PyGC方法已成功应用于几种蛋白质样品，并通过反映不同氨基酸组成的不同特殊热解图显示对分析除羊毛纤维之外的其他蛋白质也很有用。
