Chiang F T, Hsu K L, Tseng C D, Lo H M, Chern T H, Tseng Y Z
Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Republic of China.
Clin Genet. 1997 Jun;51(6):370-4. doi: 10.1111/j.1399-0004.1997.tb02493.x.
To study the association of renin gene polymorphism with essential hypertension in the Chinese population, 86 hypertensive and 107 normotensive subjects were enrolled from an epidemiologic survey. Leukocyte DNA was extracted and digested with Hind III and Bgl I restriction enzymes. Southern hybridization was done with digoxigenin-incorporated renin gene probes generated by polymerase chain reaction. The restriction fragments were detected by anti-digoxigenin antibody and enzyme methods. Two Hind III polymorphysms of the renin gene (8.7 kb and 6.2 kb) were identified. The allele frequences were 129(75%) and 43(25%), respectively, in hypertensives; they were 139(65%) and 75(35%), respectively, in normotensives (chi2 = 4.074, p = 0.044). The genotypes of 8.7/8.7,8.7/6.2 and 6.2/6.2 were significantly different between hypertensives and normotensives, being 45(52%), 39(45%), 2(3%) and 48(45%), 43(40%), and 16(15%), respectively (chi2 = 9.002, p = 0.11). The Bgl I polymorphism did not show a difference between hypertensives and normotensives. Thus, we conclude that the renin gene Hind III polymorphysm is associated with hypertension in this Chinese population.
为研究肾素基因多态性与中国人群原发性高血压的关系，从一项流行病学调查中选取了86例高血压患者和107例血压正常者。提取白细胞DNA，并用Hind III和Bgl I限制性内切酶进行消化。采用聚合酶链反应生成的地高辛标记肾素基因探针进行Southern杂交。通过抗地高辛抗体和酶法检测限制性片段。鉴定出肾素基因的两种Hind III多态性（8.7 kb和6.2 kb）。在高血压患者中，等位基因频率分别为129（75%）和43（25%）；在血压正常者中，分别为139（65%）和75（35%）（χ2 = 4.074，p = 0.044）。高血压患者和血压正常者之间8.7/8.7、8.7/6.2和6.2/6.2基因型存在显著差异，分别为45（52%）、39（45%）、2（3%）和48（45%）、43（40%）和16（15%）（χ2 = 9.002，p = 0.11）。Bgl I多态性在高血压患者和血压正常者之间未显示出差异。因此，我们得出结论，在这一中国人群中，肾素基因Hind III多态性与高血压相关。
