Schiöth H B, Petersson S, Muceniece R, Szardenings M, Wikberg J E
Department of Pharmaceutical Pharmacology, Biomedical Center, Uppsala University, Sweden.
FEBS Lett. 1997 Jun 30;410(2-3):223-8. doi: 10.1016/s0014-5793(97)00593-0.
The non-homologous N-terminal regions of four human melanocortin (MC) receptors were truncated in order to investigate their putative participation in ligand binding. Eleven constructs were made, where different numbers of residues from the N terminus were deleted. These constructs were used for transient expression experiments in COS cells and analysed by ligand binding. The results show that 27, 25, 28, and 20 amino acids could be deleted from the N terminus of the human MC1, MC3, MC4 and MC5 receptors, respectively, including all potential N-terminal glycosylation sites in the MC1 and the MC4 receptors, without affecting ligand binding or expression levels. The results indicate that the N-terminal regions of the human MC1, MC3, MC4 and MC5 receptors, do not play an important role for the ligand binding properties of these receptors.
为了研究四种人类促黑素皮质素（MC）受体的N端非同源区域是否可能参与配体结合，对其进行了截短。构建了11种构建体，从N端删除了不同数量的残基。这些构建体用于COS细胞中的瞬时表达实验，并通过配体结合进行分析。结果表明，分别可以从人类MC1、MC3、MC4和MC5受体的N端删除27、25、28和20个氨基酸，包括MC1和MC4受体中所有潜在的N端糖基化位点，而不会影响配体结合或表达水平。结果表明，人类MC1、MC3、MC4和MC5受体的N端区域对这些受体的配体结合特性不起重要作用。
