Batanero E, Ledesma A, Villalba M, Rodríguez R
Departamento de Bioquímica y Biologia Molecular, Facultad de Química,Universidad Complutense, Madrid, Spain.
FEBS Lett. 1997 Jun 30;410(2-3):293-6. doi: 10.1016/s0014-5793(97)00582-6.
The Ole e 6 allergen from olive tree pollen has been isolated by combining gel permeation and reverse-phase chromatographies. It is a single and highly acidic (pI 4.2) polypeptide chain protein. Its NH2-terminal amino acid sequence has been determined by Edman degradation. Total RNA from the olive tree pollen was isolated, and a specific cDNA was amplified by the polymerase chain reaction using a degenerate oligonucleotide primer designed according to the NH2-terminal sequence of the protein. The nucleotide sequencing of the cDNA rendered an open reading frame encoding a 50 amino acid polypeptide chain, in which two sets of the sequential motif Cys-X3-Cys-X3-Cys are present. No sequence similarity has been found between this protein and other previously described polypeptides.
通过结合凝胶渗透色谱法和反相色谱法，从橄榄树花粉中分离出了 Ole e 6 过敏原。它是一种单一的、高酸性（pI 4.2）的多肽链蛋白。其氨基末端氨基酸序列已通过埃德曼降解法测定。从橄榄树花粉中分离出总 RNA，并使用根据该蛋白氨基末端序列设计的简并寡核苷酸引物，通过聚合酶链反应扩增出特定的 cDNA。对该 cDNA 的核苷酸测序得到了一个编码 50 个氨基酸多肽链的开放阅读框，其中存在两组连续基序 Cys-X3-Cys-X3-Cys。该蛋白与其他先前描述的多肽之间未发现序列相似性。
