Hughes G J, Frutiger S, Savoy L A, Reason A J, Morris H R, Jaton J C
Dept. of Medical Biochemistry, Faculty of Medicine, University of Geneva, Medical Center, Switzerland.
FEBS Lett. 1997 Jun 30;410(2-3):443-6. doi: 10.1016/s0014-5793(97)00629-7.
The main objective of this work was to unequivocally determine the C-terminal sequence of human milk free secretory component (SC). It was found to end at arginine-585, i.e. 33 amino acids downstream from the major heterogeneous C-terminal residue previously identified for colostrum SC. In contrast, our data showed that the C-terminal end of SC was found to be homogeneous. Conflicting assignments, Asp/Gln, a missing Asn-211, Asp/Asn, Glu/Gln were corrected and found to agree with the cDNA sequence. An Ala/Val substitution at position 562 (domain VI) was identified. Its genetic significance is uncertain at present.
