White M A, Bailey J C, Cannon G C, Heinhorst S
Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg 39406-5043, USA.
FEBS Lett. 1997 Jun 30;410(2-3):509-14. doi: 10.1016/s0014-5793(97)00661-3.
A DNA polymerase was partially purified and characterized from the photosynthetic organelles (cyanelles) of the protist, Cyanophora paradoxa. While cyanelles have several cyanobacterial features, such as a lysozyme-sensitive cell wall, unstacked thylakoids and light harvesting phycobilisomes, their genome size and structure resemble those of chloroplasts, suggesting that cyanelles occupy a unique intermediate position between chloroplasts and their phylogenetic ancestors, the cyanobacteria. When comparing the biochemical characteristics of the cyanelle DNA polymerase to those of its counterparts from higher plant chloroplasts and from a cyanobacterium, it is clear that the cyanelle enzyme resembles chloroplast DNA polymerases which are eukaryotic gamma-type enzymes.
从原生生物蓝氏原绿藻（Cyanophora paradoxa）的光合细胞器（蓝小体）中部分纯化并鉴定了一种DNA聚合酶。虽然蓝小体具有一些蓝细菌的特征，如对溶菌酶敏感的细胞壁、未堆叠的类囊体和捕光藻胆体，但其基因组大小和结构类似于叶绿体，这表明蓝小体在叶绿体及其系统发育祖先蓝细菌之间占据独特的中间位置。当将蓝小体DNA聚合酶的生化特性与其来自高等植物叶绿体和蓝细菌的对应物进行比较时，很明显蓝小体酶类似于叶绿体DNA聚合酶，后者是真核γ型酶。
