Memory T cell tolerance to superantigens is not due to increased susceptibility to apoptosis.

Naive (virgin) and memory T lymphocytes differ markedly in their response to superantigens (SAg). When cultured with the SAg staphylococcal enterotoxin B (SEB), virgin but not memory CD4(+) T cells proliferate and secrete lymphokines. Memory cells do express increased levels of activation markers after interaction with SEB, which suggests that the cells are not ignorant of the SAg. In this report, we have considered whether SEB, rather than activating memory cells, promotes their death by apoptosis. Our results indicate that while in vivo exposure to SEB induces apoptosis, there is no greater level of cell death in the memory cell population relative to virgin cells. Further, elimination of the Fas-mediated cell death pathway does not permit memory cells to be stimulated by SEB. Memory T cells from either Fas-expressing or Fas-deficient (MRL-lpr/lpr) mice are hyporesponsive to SEB. Blockade of Fas/Fas-ligand interactions by a Fas-Fc chimeric protein does not permit BALB/c memory cells to proliferate upon culture with SEB. These results provide evidence that the failure of memory T cells to respond to SEB is not due to cell death and that inactivation (anergy) is the likely fate of these cells when they encounter SEB.