陆龟（红腿陆龟）β链血红蛋白的cDNA衍生氨基酸序列。

cDNA-derived amino-acid sequence of a land turtle (Geochelone carbonaria) beta-chain hemoglobin.

作者信息

Bordin S, Meza A N, Saad S T, Ogo S H, Costa F F

机构信息

Centro de Hematologia e Hemoterapia da Unicamp, Universidade Estadual de Campinas, São Paulo, Brazil.

出版信息

Biochem Mol Biol Int. 1997 Jun;42(2):255-60. doi: 10.1080/15216549700202641.

DOI:10.1080/15216549700202641

PMID:9238523

Abstract

The cDNA sequence encoding the turtle Geochelone carbonaria beta-chain was determinated. The isolation of hemoglobin mRNA was based on degenerate primers' PCR in combination with 5'- and 3'-RACE protocol. The full length cDNA is 615 bp with the ATG start codon at position 53 and TGA stop codon at position 495; The AATAAA polyadenylation signal is found at position 599. The deduced polypeptyde contains 146 amino-acid residues. The predicted amino acid sequence shares 83% identity with the beta-globin of a related specie, the aquatic turtle C. p. belli. Otherwise, identity is higher when compared with chicken beta-Hb (80%) than with other reptilian orders (Squamata, 69%, and Crocodilia, 61%). Compared with human HbA, there is 67% identity, and at least three amino acid substitutions could be of some functional significance (Glu43 beta-->Ser, His116 beta-->Thr and His143 beta-->Leu). To our knowledge this represents the first cDNA sequence of a reptile globin gene described.

摘要

确定了编码陆龟红腿陆龟β链的cDNA序列。血红蛋白mRNA的分离基于简并引物PCR结合5'-和3'-RACE方案。全长cDNA为615 bp，起始密码子ATG位于第53位，终止密码子TGA位于第495位；AATAAA多聚腺苷酸化信号位于第599位。推导的多肽包含146个氨基酸残基。预测的氨基酸序列与相关物种水龟C. p. belli的β珠蛋白有83%的同一性。此外，与鸡β-Hb（80%）相比，同一性高于与其他爬行纲目（有鳞目，69%，鳄目，61%）。与人类HbA相比，有67%的同一性，至少三个氨基酸取代可能具有一定的功能意义（Glu43β→Ser、His116β→Thr和His143β→Leu）。据我们所知，这代表了所描述的第一个爬行动物珠蛋白基因的cDNA序列。