Cheng L P, Liu W Y
Shanghai Institute of Biochemistry, Academia Sinica, China.
Biochem Mol Biol Int. 1997 Jun;42(2):381-90. doi: 10.1080/15216549700202781.
In this paper, we report more information on the important role of the aldehyde group in the sarcin/ricin domain of 28S rRNA in rat liver ribosome. We find D-amino acids, amino acid derivatives having free amino group and two polyamines can also partially restore the activity of cinnamomin-inactivated ribosomes. However, amino acid derivatives and a tripeptide with blocked amino group cannot. Neither sodium borohydride nor the L-amino acids can restore the activity of ribosomes inactivated by alpha-sarcin. These data demonstrate that partial restoration of the activity of the inactivated ribosome is indeed the result of the blockage of the aldehyde group. It reaches the conclusion that emergence of the active aldehyde group in the sarcin/ricin domain of 28S rRNA is one of factors that inactivate the ribosome for protein synthesis.
在本文中，我们报告了更多关于醛基在大鼠肝脏核糖体28S rRNA的肌动蛋白/蓖麻毒素结构域中重要作用的信息。我们发现D-氨基酸、具有游离氨基的氨基酸衍生物和两种多胺也能部分恢复肉桂霉素失活核糖体的活性。然而，氨基酸衍生物和具有封闭氨基的三肽则不能。硼氢化钠和L-氨基酸都不能恢复α-肌动蛋白失活核糖体的活性。这些数据表明，失活核糖体活性的部分恢复确实是醛基被阻断的结果。得出的结论是，28S rRNA的肌动蛋白/蓖麻毒素结构域中活性醛基的出现是使核糖体失活以进行蛋白质合成的因素之一。
