Wetprasit N, Chulavatnatol M
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Biochem Mol Biol Int. 1997 Jun;42(2):399-408. doi: 10.1080/15216549700202801.
Sugar-lectin binding assay was developed as a simple method which employed direct coating of microtiter plate with galactose-binding lectins. Biotin-galactose conjugate was used to bind to the immobilized lectins. The bound conjugate was then detected using streptavidin-horseradish peroxidase. Using the assay in conjunction with various competing carbohydrates, jackfruit lectin from Artocarpus heterophyllus was found to be specific for alpha-anomer of galactoside with an aromatic residue.
糖-凝集素结合测定法是一种简单的方法，该方法采用用半乳糖结合凝集素直接包被微量滴定板。生物素-半乳糖共轭物用于与固定化的凝集素结合。然后使用链霉亲和素-辣根过氧化物酶检测结合的共轭物。将该测定法与各种竞争性碳水化合物结合使用时，发现来自木菠萝的木菠萝凝集素对带有芳香族残基的半乳糖苷α-异头物具有特异性。
