Harada M, Suganuma N, Furuhashi M, Nagasaka T, Nakashima N, Kikkawa F, Tomoda Y, Furui K
Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Japan.
Mol Hum Reprod. 1996 May;2(5):307-15. doi: 10.1093/molehr/2.5.307.
To clarify whether apoptosis is involved in endometriosis, we obtained eutopic endometrial tissues along with endometriotic tissues from the uterus (adenomyosis) (n = 12) and from the ovary (n = 12) from patients undergoing gynaecological surgery. Apoptosis-induced DNA fragmentation was detected by the TdT-mediated dUTP-biotin nick-end labelling method, and immunostaining with a monoclonal antibody against the Fas, Le(y) or B-cell leukaemia/lymphoma-2 (bcl-2) was also performed using the same tissue section. Analysis showed that apoptosis was occurring in all the samples of ovarian endometriotic tissue but in only two of the 12 adenomyotic and in five of the 24 eutopic endometrial tissue samples. In none of these cases was apoptosis correlated with phases of the menstrual cycle. The expression of bcl-2 in the eutopic endometrial and adenomyotic tissues was limited to the proliferative phase, and was observed in only one of the 12 cases of ovarian endometriosis. Fas and Ley were expressed randomly across a wide range in both the eutopic and ectopic endometrial tissues. These results suggest that the features of ovarian endometriosis are different from those of adenomyosis and eutopic endometrium in terms of the involvement of apoptosis. In addition, the regulatory mechanism involved in ovarian endometriosis may differ from that in other endometrial cells.
为了阐明细胞凋亡是否与子宫内膜异位症有关，我们从接受妇科手术的患者子宫（子宫腺肌病）（n = 12）和卵巢（n = 12）中获取了在位子宫内膜组织以及子宫内膜异位组织。通过TdT介导的dUTP生物素缺口末端标记法检测细胞凋亡诱导的DNA片段化，并且使用同一组织切片对针对Fas、Le(y)或B细胞白血病/淋巴瘤-2（bcl-2）的单克隆抗体进行免疫染色。分析表明，细胞凋亡发生在所有卵巢子宫内膜异位组织样本中，但在12例子宫腺肌病样本中仅2例出现，在24例在位子宫内膜组织样本中仅5例出现。在所有这些病例中，细胞凋亡均与月经周期各阶段无关。在位子宫内膜和子宫腺肌病组织中bcl-2的表达仅限于增殖期，并且在12例卵巢子宫内膜异位症病例中仅1例观察到。Fas和Le(y)在在位和异位子宫内膜组织中均广泛随机表达。这些结果表明，就细胞凋亡的参与情况而言，卵巢子宫内膜异位症的特征不同于子宫腺肌病和在位子宫内膜。此外，卵巢子宫内膜异位症涉及的调节机制可能与其他子宫内膜细胞不同。
